anti-Schwein Adducin 2 (Beta) Antikörper für Western Blotting

Recommended Adducin 2 (Beta) Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Adducin 2 (Beta) (ADD2) Antikörper
  • si:dkey-202n14.5
  • ADDB
  • 2900072M03Rik
  • add97
  • beta-ADD
  • adducin 2
  • adducin 2 (beta)
  • ADD2
  • add2
  • Add2
Epitop
Middle Region
7
3
3
2
2
2
2
1
1
1
1
1
1
1
Reaktivität
Rind (Kuh), Hund, Meerschweinchen, Pferd, Human, Maus, Schwein, Kaninchen, Ratte (Rattus)
59
36
34
2
2
1
1
1
Wirt
Kaninchen
57
3
Klonalität
Polyklonal
Konjugat
Unkonjugiert
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (IF), Western Blotting (WB)
41
20
13
10
9
7
5
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN2784900
$ 289.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN1891523 IP WB Rabbit IgG AA 1-50 Anmelden zum Anzeigen Polyclonal 0

General

Antigen Adducin 2 (Beta) (ADD2) Antikörper
Epitop Middle Region
(7), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Rind (Kuh), Hund, Meerschweinchen, Pferd, Human, Maus, Schwein, Kaninchen, Ratte (Rattus)
(59), (36), (34), (2), (2), (1), (1), (1)
Wirt Kaninchen
(57), (3)
Klonalität
Polyklonal
Konjugat Unkonjugiert
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunofluorescence (IF), Western Blotting (WB)
(41), (20), (13), (10), (9), (7), (5), (1), (1)
Pubmed 1 Publikation vorhanden
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Produktdetails

Antigendetails Anwendungsinformationen Handhabung Referenzen Bilder
Blocking Peptide Zu diesem Produkt passt das Blocking Peptide ABIN972930.
Sequenz VVNGREEEQT AEEILSKGLS QMTTSADTDV DTSKDKTESV TSGPMSPEGS
Homologie Cow: 100%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 79%, Pig: 100%, Rabbit: 93%, Rat: 100%
Produktmerkmale This is a rabbit polyclonal antibody against ADD2. It was validated on Western Blot using a cell lysate as a positive control.
Reinigung Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human ADD2

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Referenzen Bilder zurück nach oben
Antigen
Andere Bezeichnung ADD2 (ADD2 Antibody Abstract)
Hintergrund Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Various adducin beta mRNAs, alternatively spliced at 3'end and/or internally spliced and encoding different isoforms, have been described. The functions of all the different isoforms are not known.
Alias Symbols: ADDB
Protein Interaction Partner: UBC, APP, RPH3A, PRKACA, PRKCD, PTPRZ1, FYN, CALM1, ADD2, ADD1,
Protein Size: 726
Molekulargewicht 80 kDa
Gen-ID 119
NCBI Accession NM_001617, NP_001608
UniProt P35612
Pathways Regulation of Actin Filament Polymerization

Anwendungsinformationen

Produktdetails Antigendetails Handhabung Referenzen Bilder zurück nach oben
Applikations-hinweise Optimal working dilutions should be determined experimentally by the investigator.
Kommentare

Antigen size: 726 AA

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Antigendetails Anwendungsinformationen Referenzen Bilder zurück nach oben
Format Liquid
Konzentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -20 °C
Informationen zur Lagerung For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen

Produktdetails Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Kato, Miyata, Tabara, Katsuya, Yanai, Hanada, Kamide, Nakura, Kohara, Takeuchi, Mano, Yasunami, Kimura, Kita, Ueshima, Nakayama, Soma, Hata, Fujioka, Kawano, Nakao, Sekine, Yoshida, Nakamura, Saruta, Ogihara, Sugano, Miki, Tomoike: "High-density association study and nomination of susceptibility genes for hypertension in the Japanese National Project." in: Human molecular genetics, Vol. 17, Issue 4, pp. 617-27, 2008

Bilder

Produktdetails Antigendetails Anwendungsinformationen Handhabung Referenzen zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Adducin 2 (Beta) (ADD2) (Middle Region) antibody (ABIN2784900) WB Suggested Anti-ADD2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
 image for anti-Adducin 2 (Beta) (ADD2) (Middle Region) antibody (ABIN2784900) anti-Adducin 2 (Beta) (ADD2) (Middle Region) antibody (Image 2)