TNF alpha Protein

Produktnummer ABIN988271

Dieses Recombinant TNF alpha-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für TNF alpha Protein (ABIN988271)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Reinheit: > 97 % by SDS-PAGE and HPLC analyses.

Produktdetails

Sequenz: MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIA

Produktmerkmale: Fully biologically active when compared to standard. The ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is < 0.1 ng/ml, corresponding to a specific activity of > 1.0×107 units/mg.

Endotoxin-Niveau: Level Less than 1EU/µg of rMuTNF-alpha as determined by LAL method

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at

Lagerung: 4 °C

Antigendetails

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: Tumor Necrosis Factor-alpha (TNF-alpha)

Hintergrund: Tumor necrosis factor alpha (TNF-alpha) is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. Mouse TNF-alpha occurs as a membrane-anchored form. The naturally-occurring form of TNF-alpha is glycosylated, but non-glycosylated recombinant TNF-alpha has comparable biological activity. The biologically active native form of TNF-alpha is reportedly a trimer. Human and mouse TNF-alpha show approximately 79% homology at the amino acid level and crossreactivity between the two species. Synonym: Tumor Necrosis Factor-alpha ( TNF-alpha), Mouse. Formulation: Lyophilized from a 0.2µm filtered solution in PBS, pH 7.2.

Molekulargewicht: Approximately 17.3 kDa. The recombinant mouse TNF-alpha is a soluble 157 amino acid protein which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome