TNF alpha Protein

Produktnummer ABIN935843

Dieses Recombinant TNF alpha-Protein wird in Escherichia coli (E. coli) produziert. Es ist 1 Publikation verfügbar.

Kurzübersicht für TNF alpha Protein (ABIN935843)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 98 % pure

Produktdetails

Sequenz: VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL

Produktmerkmale: Purified recombinant Human TNF alpha protein
Expression System: E.coli
Bioactivity: The ED50 as determined by the cytolysis of Mouse L929 cells in the presence of Actinomycin D is ? 0.05 ng/mL, corresponding to a specific activity of ? 2 x 10^7 units/mg.

Endotoxin-Niveau: < 0.1 ng per μg (1 EU/μg).

Anwendungsinformationen

Applikationshinweise: Each Investigator should determine their own optimal working dilution for specific applications.

Kommentare:

Biological activity: The ED50 as determined by the cytolysis of Mouse L929 cells in the presence of Actinomycin D is approx. 0.05 ng/mL, corresponding to a specific activity of approx. 2 x 10^7 units/mg.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in water to a concentration of 0.1 - 1.0 mg/mL. Allow to sit at room temperature for 2 hours before use.

Buffer: Lyophilized from 3 mM Tris, pH 8.0.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.

Referenzen

Spatz, Eibl, Hink, Wolf, Fischer, Mayr, Schernthaner, Eibl: "Impaired primary immune response in type-1 diabetes. Functional impairment at the level of APCs and T-cells." in: Cellular immunology, Vol. 221, Issue 1, pp. 15-26, (2003) (PubMed).

Antigendetails

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: TNF alpha

Hintergrund: TNF-alpha is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes.
Alternative Names: TNFSF2 protein, Necrosin protein, TNF-a protein, Cachectin protein, Cytotoxin protein, TNFa protein, DIF protein, Tumor Necrosis Factor protein, TNF-alpha protein, Differentiation-inducing factor protein

Molekulargewicht: 17.4 kDa

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome