TNF alpha Protein (full length)

Produktnummer ABIN935842

Produktdetails

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: full length

Spezies: Human

Quelle: Escherichia coli (E. coli)

Produktmerkmale: Purified recombinant Human TNF alpha protein
Expression System: E.coli
Bioactivity: The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is

Aufreinigung: Proprietary chromatographic technique

Reinheit: > 98 % pure

Anwendungsinformationen

Applikationshinweise: Each Investigator should determine their own optimal working dilution for specific applications.

Testdurchführung:

Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 1.234 as the extinction coefficient for a 0.1 % (1 mg/mL) solution.
2. Analysis by RP-HPLC, using a calibrated solution of TNF-a as a Reference Standard.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in water to a concentration of 0.1 - 1.0 g/mL.

Buffer: Lyophilized from 20 mM PBS, pH 7.2, with 10 mM NaCl.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.

Referenzen

Spatz, Eibl, Hink, Wolf, Fischer, Mayr, Schernthaner, Eibl: "Impaired primary immune response in type-1 diabetes. Functional impairment at the level of APCs and T-cells." in: Cellular immunology, Vol. 221, Issue 1, pp. 15-26, (2003) (PubMed).

Antigendetails

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: TNF alpha (TNF alpha Produkte)

Hintergrund: Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages. TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells. Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.
Alternative Names: Tumor Necrosis Factor protein, TNFa protein, Cytotoxin protein, DIF protein, TNFSF2 protein, TNF-alpha protein, TNF-a protein, Differentiation-inducing factor protein, Necrosin protein, Cachectin protein

Molekulargewicht: 17,483 Da

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome