PPIL1 Protein (AA 1-166) (His tag)

Produktnummer ABIN935000

Produktdetails

Target: PPIL1 (Peptidylprolyl Isomerase (Cyclophilin)-Like 1 (PPIL1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 1-166

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses PPIL1 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MAAIPPDSWQ PPNVYLETSM GIIVLELYWK HAPKTCKNFA ELARRGYYNG TKFHRIIKDF MIQGGDPTGT GRGGASIYGK QFEDELHPDL KFTGAGILAM ANAGPDTNGS QFFVTLAPTQ WLDGKHTIFG RVCQGIGMVN RVGMVETNSQ DRPVDDVKII KAYPSGLEHH HHHH

Produktmerkmale: Purified recombinant Human PPIL1 protein
Expression System: E.coli
Bioactivity: Specific activity is > 155 nM/min/µg, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 °C in Tris-Hcl pH 8.0 using chymotrypsin.
Molecular weight on SDS-PAGE will appear higher.

Reinheit: > 95 % pure

Anwendungsinformationen

Applikationshinweise: PPIL1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Testdurchführung:

1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA).
   2. Add recombinant PPIL1 protein with various concentrations (1 µg, 1 µg, 1 µg) in assay buffer.
   3. Mix by inversion and equilibrate to 1 °C and monitor the A410 nm until the value is constant using a spectrophotometer.
   4. Add pre-chilled chymotrypsin to 12.5 µM and mix immediately.
   5. Record the increase in A410 nm for 20 minutes

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Supplied as a liquid in 20 mM Tris-HCl, pH 8.0, containing 20 % glycerol.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: RT/-20 °C

Informationen zur Lagerung: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Antigendetails

Target: PPIL1 (Peptidylprolyl Isomerase (Cyclophilin)-Like 1 (PPIL1))

Andere Bezeichnung: PPIL1 (PPIL1 Produkte)

Synonyme: CYPL1 Protein, PPIase Protein, hCyPX Protein, MGC79515 Protein, PPIL1 Protein, 1110060O10Rik Protein, AI327391 Protein, Cypl1 Protein, zgc:123245 Protein, peptidylprolyl isomerase like 1 Protein, peptidylprolyl isomerase like 1 L homeolog Protein, peptidyl-prolyl cis-trans isomerase-like 1 Protein, peptidylprolyl isomerase (cyclophilin)-like 1 Protein, PPIL1 Protein, ppil1.L Protein, ppil1 Protein, LOC707647 Protein, Ppil1 Protein

Hintergrund: Peptidylprolyl isomerase (cyclophilin)-like 1, also known as PPIL1, is a member of peptidyl-propyl cis-trans isomerase (PPIase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. Human PPIL1 might play an important role in proliferation of cancer cells through modulation of phosphorylation of stathmin. So it is expected as a novel molecular target for colon-cancer therapy. Recombinant PPIL1, fused to His-tag at C-terminus, was expressed in E. coli and purified by conventional chromatography techniques.
Alternative Names: PPIL 1, Cyclophilin related gene 1 protein, PPIL-1, Peptidylprolyl isomerase (cyclophilin)-like 1 CGI124 protein, Peptidylprolyl isomerase (cyclophilin) like 1 protein, CYPL1 protein, PPIL-1 protein, rotamase0., PPIL1, PPIL 1 protein, PPIase protein, PPIL1 protein, peptidylprolyl isomerase like 1 protein, Peptidylprolyl isomerase (cyclophilin)-like 1 protein, CGI-124 protein, MGC678 protein, hCyPX protein

Molekulargewicht: 19.3 kDa (174 AA)