TNF alpha Protein (AA 80-235)

Produktnummer ABIN7607268

Recombinant TNF alpha-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für TNF alpha Protein (AA 80-235) (ABIN7607268)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Immunogen (Imm), Activity Assay (AcA)

Reinheit: >90 % as determined by SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 80-235

Verwendungszweck: Recombinant Mouse TNFa/TNF-alpha Protein, No tag (Active)

Endotoxin-Niveau: <1 EU/μg of the protein by the LAL method.

Biological Activity Comment: Recombinant Mouse TNFa/TNF-alpha (Cat #: AMB94401) induces cytotoxicity in the mouse fibroblast cells (L-929) in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 29-116 pg/mL, corresponding to a specific activity of 8.62x10^6-3.45x10^7 units/mg.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Haltbarkeit: 12 months

Antigendetails

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: Tumor necrosis factor

Hintergrund: Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor, membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2

UniProt: P06804

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome