TNF alpha Protein (AA 78-234)

Produktnummer ABIN7677802

Recombinant TNF alpha-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für TNF alpha Protein (AA 78-234) (ABIN7677802)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Rind (Kuh)

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS), Activity Assay (AcA)

Reinheit: > 90 %

Produktdetails

Proteineigenschaft: AA 78-234

Sequenz: MLRSSSQASS NKPVAHVVAD INSPGQLRWW DSYANALMAN GVKLEDNQLV VPADGLYLIY SQVLFRGQGC PSTPLFLTHT ISRIAVSYQT KVNILSAIKS PCHRETPEWA EAKPWYEPIY QGGVFQLEKG DRLSAEINLP DYLDYAESGQ VYFGIIAL

Aufreinigung: > 90% by SDS-PAGE

Endotoxin-Niveau: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 15ng/ml.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate-Buffered Saline ( pH 7.4) containing 10 % glycerol

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: TNF-alpha

Hintergrund: TNF-alpha, also known as tumor necrosis factor, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. This protein is produced by a wide variety of immune and epithelial cell types. It is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface. Cell surface TNF can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. Recombinant Bovine TNF-alpha was expressed in E. coli and purified by using conventional chromatography techniques.

Molekulargewicht: 17.5 kDa (158aa) confirmed by MALDI-TOF

NCBI Accession: NP_776391

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome