TNF alpha Protein

Produktnummer ABIN7539350

Dieses Recombinant TNF alpha-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für TNF alpha Protein (ABIN7539350)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 % by SDS-PAGE and RP-HPLC analysis

Produktdetails

Verwendungszweck: TNF-alpha

Sequenz: MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA E AKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL

Spezifität: Chromosomal location:6p21.3

Produktmerkmale: Length (aa):158

Anwendungsinformationen

Applikationshinweise: The ED50 as determined by the proliferation assay with TF-1 cells is in the range of 0.05-0.4 ng/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: The lyophilized TNF-alpha is soluble in water and most aqueous buffers. The lyophilized powder can be reconstituted in sterile water to a concentration of 0.1 mg/mL. This solution can be diluted into other buffered solutions or stored at -20 °C for future use.

Buffer: 20 mM PB, ph 7.2 + 100 mM NaCl

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Lyophilized Tumor Necrosis Factor-alpha although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-alpha should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Antigendetails

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: TNF-alpha

Hintergrund: TNF, DIF, TNFA, TNFSF2, TNF-alpha,Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages. TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells. Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer. Human recombinant Tumor Necrosis Factor-α produced in E. coli is a single, non-glycosylated, polypeptide chain containing 158 amino acids and having a molecular mass of 17483.77 Dalton.

Molekulargewicht: 17.5 kDa

Gen-ID: 7124

NCBI Accession: NM_000594, NP_000585

UniProt: P01375

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome