Osteoprotegerin Protein (Fc Tag,His tag)

Produktnummer ABIN7534823

Produktdetails

Target: Osteoprotegerin (TNFRSF11B) (Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses Osteoprotegerin Protein ist gelabelt mit Fc Tag,His tag.

Verwendungszweck: Active Recombinant Mouse TNFRSF11B/Osteoprotegerin Protein

Sequenz: MNKWLCCALL VLLDIIEWTT QETLPPKYLH YDPETGHQLL CDKCAPGTYL KQHCTVRRKT LCVPCPDHSY TDSWHTSDEC VYCSPVCKEL QSVKQECNRT HNRVCECEEG RYLEIEFCLK HRSCPPGSGV VQAGTPERNT VCKKCPDGFF SGETSSKAPC IKHTNCSTFG LLLIQKGNAT HDNVCSGNRE ATQKCGIDVT LCEEAFFRFA VPTKIIPNWL SVLVDSLPGT KVNAESVERI KRRHSSQEQT FQLLKLWKHQ NRDQEMVKKI IQDIDLCESS VQRHLGHSNL TTEQLLALME SLPGKKISPE EIERTRKTCK SSEQLLKLLS LWRIKNGDQD TLKGLMYALK HLKTSHFPKT VTHSLRKTMR FLHSFTMYRL YQKLFLEMIG NQVQSVKISC L

Spezifität: Met1-Leu401

Reinheit: > 87 % by SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: 1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human TNFSF11 at 2 μg/mL (100 μL/well) can bind Recombinant Mouse TNFRSF11B with a linear range of 3-10 ng/mL.|2.Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL. The ED₅₀ for this effect is 0.13-0.54 ng/mL in the presence of 20 ng/mL Recombinant Human TRAIL/TNFSF10.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Antigendetails

Target: Osteoprotegerin (TNFRSF11B) (Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B))

Andere Bezeichnung: TNFRSF11B/Osteoprotegerin (TNFRSF11B Produkte)

Synonyme: CD254 Protein, ODF Protein, OPGL Protein, OPTB2 Protein, RANKL Protein, TRANCE Protein, hRANKL2 Protein, sOdf Protein, OCIF Protein, OPG Protein, TR1 Protein, Opg Protein, ocif Protein, opg Protein, tr1 Protein, TNF superfamily member 11 Protein, TNF receptor superfamily member 11b Protein, tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin) Protein, TNF receptor superfamily member 11B Protein, tumor necrosis factor receptor superfamily member 11b S homeolog Protein, tumor necrosis factor receptor superfamily, member 11b Protein, TNFSF11 Protein, TNFRSF11B Protein, Tnfrsf11b Protein, tnfrsf11b.S Protein

Substanzklasse: Chemical

Hintergrund: TNFRSF11B,OCIF,OPG,Osteoprotegerin,TR1,TNFRSF11B

Gen-ID: 18383

UniProt: O08712