TXN Protein (His tag)

Produktnummer ABIN7533791

Produktdetails

Target: TXN (Thioredoxin (TXN))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses TXN Protein ist gelabelt mit His tag.

Verwendungszweck: Active Recombinant Human Thioredoxin/SASP/TXN Protein

Sequenz: VKQIESKTAF QEALDAAGDK LVVVDFSATW CGPCKMIKPF FHSLSEKYSN VIFLEVDVDD CQDVASECEV KCMPTFQFFK KGQKVGEFSG ANKEKLEATI NELV

Spezifität: Val2-Val105

Reinheit: > 95 % by SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: 1.Measured by its binding ability in a functional ELISA. Immobilized Human TXN Protein at 1 μg/mL (100 μL/well) can bind TXN Rabbit mAb with a linear range of 0.976-5.3 ng/mL.|2.Measured by its ability to catalyze the reduction of insulin. The reaction leads to precipitation, which can be measured by absorbance at 650 nm. The specific activity is >9 A650/min/mg.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Antigendetails

Target: TXN (Thioredoxin (TXN))

Andere Bezeichnung: Thioredoxin/SASP/TXN (TXN Produkte)

Synonyme: TRDX Protein, TRX Protein, TRX1 Protein, ADF Protein, AW550880 Protein, Trx1 Protein, Txn Protein, trx Protein, TXN Protein, SCM1.18 Protein, trxA3 Protein, thioredoxin Protein, TR-1 Protein, Trx-1 Protein, thioredoxin Protein, thioredoxin 1 Protein, thioredoxin L homeolog Protein, similar to 44RRORF109c hypothetical protein; similar to bacterial thioredoxin (SwissProt P06544) Protein, thioredoxin pseudogene Protein, cytosolic thioredoxin Trx1 Protein, TXN Protein, Txn1 Protein, txn.L Protein, txn Protein, trx Protein, SCO0885 Protein, PHG25ORF099c Protein, LOC100349046 Protein, Txn Protein, trx1 Protein

Hintergrund:

Description: Thioredoxin, also known as ATL-derived factor, Surface-associated sulphydryl protein, SASP and TXN, is a nucleus, cytoplasm and secreted protein which belongs to the thioredoxin family. Trx-1 is the only extracellular occurring thioredoxin, and is secreted by lymphocytes, hepatocytes, fibroblasts, and several tumor cells. Plasma concentrations of Trx-1 are up to 6 nM . In cells, Trx-1 is localized predominantly in the cytoplasm. Small amounts have been detected in the nucleus and in association with the outside surface of the cells. Biological functions of Trx-1 include growth factor activity, antioxidant properties, a cofactor that provides reducing equivalents, and transcriptional regulation.

Name: TRDX, TRX, TRX1,TXN,TRX,TRX1

Gen-ID: 7295

UniProt: P10599

Pathways: Carbohydrate Homeostasis, Cell RedoxHomeostasis