Galectin 9 Protein (His tag)

Produktnummer ABIN7534733

Produktdetails

Target: Galectin 9 (LGALS9) (Lectin, Galactoside-Binding, Soluble, 9 (LGALS9))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses Galectin 9 Protein ist gelabelt mit His tag.

Verwendungszweck: Active Recombinant Human Galectin-9/LGALS9 Protein

Sequenz: AFSGSQAPYL SPAVPFSGTI QGGLQDGLQI TVNGTVLSSS GTRFAVNFQT GFSGNDIAFH FNPRFEDGGY VVCNTRQNGS WGPEERKTHM PFQKGMPFDL CFLVQSSDFK VMVNGILFVQ YFHRVPFHRV DTISVNGSVQ LSYISFQPPG VWPANPAPIT QTVIHTVQSA PGQMFSTPAI PPMMYPHPAY PMPFITTILG GLYPSKSILL SGTVLPSAQR FHINLCSGNH IAFHLNPRFD ENAVVRNTQI DNSWGSEERS LPRKMPFVRG QSFSVWILCE AHCLKVAVDG QHLFEYYHRL RNLPTINRLE VGGDIQLTHV QT

Spezifität: Ala2-Thr323

Reinheit: > 92 % by SDS-PAGE.

Sterilität: 0.22 μm filtered

Biological Activity Comment: 1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Galectin-9 at 2 μg/mL can bind Recombinant Human TIM-3 with a linear range of 5-15 μg/mL.|2.Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8x104 cells/well are added to LGALS9 coated plates (5 μg/mL and 100 μL/well) in the ppresence of 10μg/ml PHA, approximately 70-80% cells will adhere specifically after 60 minutes at 37°C.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Antigendetails

Target: Galectin 9 (LGALS9) (Lectin, Galactoside-Binding, Soluble, 9 (LGALS9))

Andere Bezeichnung: Galectin-9/LGALS9 (LGALS9 Produkte)

Synonyme: huat Protein, lgals9a Protein, ecalectin Protein, galectin-9 Protein, Lgals9 Protein, wu:fd20c09 Protein, zgc:111833 Protein, LGALS9 Protein, AA407335 Protein, AI194909 Protein, AI265545 Protein, LGALS35 Protein, Lgals5 Protein, gal-9 Protein, HUAT Protein, LGALS9A Protein, UAT Protein, UATP.I Protein, galectin 9 L homeolog Protein, galectin 9 Protein, lectin, galactoside-binding, soluble, 9 (galectin 9)-like 3 Protein, lectin, galactose binding, soluble 9 Protein, lgals9.L Protein, lgals9 Protein, lgals9l3 Protein, LGALS9 Protein, Lgals9 Protein

Hintergrund: LGALS9,HUAT,LGALS9A

Gen-ID: 3965

UniProt: O00182-2