eGFP Protein (His tag)
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- Target Alle eGFP Produkte
- eGFP (Enhanced Green Fluorescent Protein (eGFP))
- Protein-Typ
- Recombinant
- Spezies
- Aequorea victoria
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses eGFP Protein ist gelabelt mit His tag.
- Verwendungszweck
- Recombinant Aequorea victoria EGFP Protein
- Sequenz
- MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT LVTTLTYGVQ CFSRYPDHMK QHDFFKSAMP EGYVQERTIF FKDDGNYKTR AEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNYNSHN VYIMADKQKN GIKVNFKIRH NIEDGSVQLA DHYQQNTPIG DGPVLLPDNH YLSTQSALSK DPNEKRDHMV LLEFVTAAGI TLGMDELYK
- Spezifität
- Met1-Lys238(F64L,S65T)
- Reinheit
- > 95 % by SDS-PAGE.
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- <0.1EU/μg
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Enhanced Green Fluorescent Protein (eGFP) protein (His tag)
eGFP Spezies: E. coli Wirt: Escherichia coli (E. coli) >80 %
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- Buffer
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
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- Target
- eGFP (Enhanced Green Fluorescent Protein (eGFP))
- Andere Bezeichnung
- EGFP (eGFP Produkte)
- Hintergrund
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Description: GFP, also known as Green Fluorescent Protein, is a protein produced by the jellyfish (Aequorea Victoria) that produces bioluminescence in the green zone of the noticeable spectrum. Green Fluorescent Protein is a useful and ubiquitous instrument for producing chimeric proteins, where it functions as a fluorescent protein tag. GFP is expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. Green Fluorescent Protein permits a broad range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Enhanced GFP (eGFP) has F64L and S65T mutations, which make GFP show increased fluorescence and fold more efficiently under 37 °C.
Name: Green fluorescent protein,GFP,EGFP
- UniProt
- P42212
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