HLA-A*02:01 (Monomer) protein (Biotin,MAGE-A3)
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- Target
- HLA-A*02:01
- Protein-Typ
- Recombinant
- Proteineigenschaft
- Monomer
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Spezies
- Human
-
Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Biotin,MAGE-A3
- Verwendungszweck
- Biotinylated Human HLA-A*02:01 & B2M & MAGE-A3 (KVAELVHFL) Monomer Protein
- Sequenz
- Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and KVAELVHFL peptide
- Spezifität
- Uni-Prot: A0A140T913 (HLA-A*02:01), P61769 (B2M), KVAELVHFL
- Produktmerkmale
- Recombinant Biotinylated Human HLA-A*02:01 & B2M & MAGE-A3 (KVAELVHFL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and KVAELVHFL peptide.
- Reinheit
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Less than 1EU per μg by the LAL method.
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-
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Haltbarkeit
- 12 months
-
- Target
- HLA-A*02:01
- Hintergrund
- Melanoma antigen family A, 3 (MAGE-A3) is a cancer-testis antigen whose expression has been demonstrated in a wide array of malignancies including melanoma, brain, breast, lung and ovarian cancer. In addition, its ability to elicit spontaneous humoral and cellular immune responses has been shown in cancer patients.
- Molekulargewicht
- 50.50 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
- UniProt
- A0A140T913
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