IL-27 Protein (AA 21-229) (His tag)

Produktnummer ABIN7454152

Produktdetails

Target: IL-27 (IL27) (Interleukin 27 (IL27))

Protein-Typ: Recombinant

Proteineigenschaft: AA 21-229

Spezies: Human

Quelle: CHO Cells

Aufreinigungstag / Konjugat: Dieses IL-27 Protein ist gelabelt mit His tag.

Verwendungszweck: Human IL-27 Protein

Sequenz: Arg21-Lys229 (IL-27B) & Phe29-Pro243 (IL-27A)

Spezifität: Uni-Prot: Q14213 (IL-27B), Q8NEV9 (IL-27A)

Produktmerkmale: Recombinant Human IL-27 Protein is expressed from CHO with His tag at the C-Terminus.It contains Arg21-Lys229(IL-27B) & Phe29-Pro243(IL-27A).

Reinheit: > 95 % as determined by Tris-Bis PAGE

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-27 RA, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 16.8ng/ml determined by ELISA (QC Test). The affinity constant of 3.58 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS, 200 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: IL-27 (IL27) (Interleukin 27 (IL27))

Andere Bezeichnung: IL-27 (IL27 Produkte)

Synonyme: IL-27 Protein, IL-27A Protein, IL27A Protein, IL27p28 Protein, IL30 Protein, p28 Protein, RGD1561420 Protein, IL27 Protein, IL-27p28 Protein, Il30 Protein, interleukin 27 Protein, IL27 Protein, Il27 Protein

Hintergrund: Interleukin (IL)-27, a member of the IL-6/IL-12 family, has an important role in modulating inflammation in partnership with innate and adaptive immune cells.

Molekulargewicht: 50.6 kDa. Due to glycosylation, the protein migrates to 54-64 kDa based on Tris-Bis PAGE result.

UniProt: Q14213