TREM2 Protein (AA 19-174) (Fc Tag)

Produktnummer ABIN7275789

Produktdetails

Target: TREM2 (Triggering Receptor Expressed On Myeloid Cells 2 (TREM2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 19-174

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses TREM2 Protein ist gelabelt mit Fc Tag.

Verwendungszweck: Human TREM2 Protein

Sequenz: His19-Ser174

Produktmerkmale: Recombinant Human TREM2 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains His19-Ser174.

Reinheit: > 95 % as determined by Tris-Bis PAGE

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human TREM2, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-TREM2 Antibody, hFc Tag with the EC50 of 25ng/ml determined by ELISA. The affinity constant of 1.004 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS, 100 mM Arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: TREM2 (Triggering Receptor Expressed On Myeloid Cells 2 (TREM2))

Andere Bezeichnung: TREM2 (TREM2 Produkte)

Synonyme: TREM-A1 Protein, TREM-B1 Protein, TREM2 Protein, trem-2 Protein, trem2a Protein, trem2b Protein, trem2c Protein, TREM-2 Protein, Trem2a Protein, Trem2b Protein, Trem2c Protein, triggering receptor expressed on myeloid cells 2 Protein, trem-like transcript 2 protein Protein, TREM2 Protein, trem2 Protein, LOC100228399 Protein, Trem2 Protein

Hintergrund: TREM-2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa type I transmembrane member of the TREM family and Ig superfamily. Mature human TREM-2  consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. TREM-2 forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding (PubMed:10799849). Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia.

Molekulargewicht: 44.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.