SIGLEC10 Protein (AA 17-546) (His tag)

Produktnummer ABIN7275592

Produktdetails

Target: SIGLEC10 (Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10))

Protein-Typ: Recombinant

Proteineigenschaft: AA 17-546

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses SIGLEC10 Protein ist gelabelt mit His tag.

Verwendungszweck: Human Siglec-10 Protein

Sequenz: Met17-Thr546

Produktmerkmale: Recombinant Human Siglec-10 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met17-Thr546.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Siglec-10, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-Siglec-10 Ab., hFc Tag with the EC50 of 7.4ng/ml determined by ELISA. The affinity constant of 15.9μM as determined in SPR assay (Biacore T200). See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in 20 mM PB, 150 mM NaCl, 25 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: SIGLEC10 (Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10))

Andere Bezeichnung: Siglec-10 (SIGLEC10 Produkte)

Synonyme: PRO940 Protein, SIGLEC-10 Protein, SLG2 Protein, Siglecg Protein, SIGLEC10 Protein, slg2 Protein, pro940 Protein, siglec-10 Protein, sialic acid binding Ig like lectin 10 Protein, sialic acid binding Ig-like lectin 10 Protein, sialic acid-binding Ig-like lectin 10 Protein, SIGLEC10 Protein, Siglec10 Protein, LOC484343 Protein, LOC100066644 Protein, siglec10 Protein, LOC100513863 Protein, LOC100340374 Protein

Hintergrund: Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.

Molekulargewicht: 59.2 kDa. Due to glycosylation, the protein migrates to 70-79 kDa based on Tris-Bis PAGE result.