RNF34 Protein (AA 24-197) (Fc Tag)
-
- Target Alle RNF34 Proteine anzeigen
- RNF34 (Ring Finger Protein 34 (RNF34))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 24-197
-
Spezies
- Maus
-
Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses RNF34 Protein ist gelabelt mit Fc Tag.
- Verwendungszweck
- Mouse RNF43 Protein
- Sequenz
- Gly24-Tyr197
- Produktmerkmale
- Recombinant Mouse RNF43 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly24-Tyr197.
- Reinheit
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Mouse R spondin 1, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.21μg/ml determined by ELISA. See testing image for detail.
-
-
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Lyophilized
- Rekonstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Haltbarkeit
- 12 months
-
- Target
- RNF34 (Ring Finger Protein 34 (RNF34))
- Andere Bezeichnung
- RNF34 (RNF34 Produkte)
- Synonyme
- CARP-1 Protein, CARP1 Protein, RFI Protein, RIF Protein, RIFF Protein, hRFI Protein, rnf34 Protein, AW061037 Protein, AW536122 Protein, BC004042 Protein, C88279 Protein, Momo Protein, fi35g06 Protein, wu:fi35g06 Protein, zgc:114043 Protein, zgc:171755 Protein, ring finger protein 34 Protein, E3 ubiquitin-protein ligase RNF34 Protein, ring finger protein 34a Protein, ring finger protein 34b Protein, Residual feed intake Protein, RNF34 Protein, rnf34 Protein, Rnf34 Protein, rnf34a Protein, rnf34b Protein, RFI Protein
- Hintergrund
- RNF43 (E3 ubiquitin-protein ligase RNF43 or RING-type E3 ubiquitin transferase RNF43) functions as a tumor suppressor, by exerting a predominant negative feedback mechanism in the Wnt/β-catenin signaling pathway. RNF43 inhibits Wnt/beta-catenin signaling by ubiquitinating Frizzled receptor and targeting it to the lysosomal pathway for degradation.
- Molekulargewicht
- 45.6 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result.
- UniProt
- Q5NCP0
-