PVRL4 Protein (AA 32-351) (His-Avi Tag,Biotin)

Produktnummer ABIN7275314

Produktdetails

Target: PVRL4 (Poliovirus Receptor-Related 4 (PVRL4))

Protein-Typ: Recombinant

Proteineigenschaft: AA 32-351

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses PVRL4 Protein ist gelabelt mit His-Avi Tag,Biotin.

Verwendungszweck: Biotinylated Human Nectin-4 Protein

Sequenz: Gly32-Val351

Produktmerkmale: Recombinant Biotinylated Human Nectin-4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly32-Val351.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human Nectin-4, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Nectin4 Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: PVRL4 (Poliovirus Receptor-Related 4 (PVRL4))

Andere Bezeichnung: Nectin-4 (PVRL4 Produkte)

Synonyme: PVRL4 Protein, si:ch211-155e24.1 Protein, 1200017F15Rik Protein, Prr4 Protein, RGD1559826 Protein, EDSS1 Protein, LNIR Protein, PRR4 Protein, nectin-4 Protein, nectin cell adhesion molecule 4 Protein, NECTIN4 Protein, nectin4 Protein, Nectin4 Protein

Hintergrund: Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus.

Molekulargewicht: 37.2 kDa. Due to glycosylation, the protein migrates to 46-48 kDa based on Tris-Bis PAGE result.

UniProt: Q96NY8

Pathways: Cell-Cell Junction Organization