Dieses PVRL4 Protein ist gelabelt mit His-Avi Tag.
Verwendungszweck
Human Nectin-4 Protein
Sequenz
Gly32-Val351
Produktmerkmale
Recombinant Human Nectin-4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly32-Val351.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by SEC-HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human Nectin-4 at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Nectin4 Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA. See testing image for detail.
PVRL4
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
PVRL4
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
Active
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus.
Molekulargewicht
37.2 kDa. Due to glycosylation, the protein migrates to 46-48 kDa based on Tris-Bis PAGE result.