PVRL1
(Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1))
Protein-Typ
Recombinant
Proteineigenschaft
AA 31-334
Spezies
Human
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses PVRL1 Protein ist gelabelt mit His-Avi Tag,Biotin.
Verwendungszweck
Biotinylated Human Nectin-1/PVRL1/CD111 Protein
Sequenz
Gln31-Thr334
Produktmerkmale
Recombinant Biotinylated Human Nectin-1/PVRL1/CD111 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln31-Thr334.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
The affinity constant of 22.83 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Crystallography grade
PVRL1
Spezies: Human
Wirt: Escherichia coli (E. coli)
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, SDS, ELISA, Crys
Crystallography grade
PVRL1
Spezies: Human
Wirt: Insektenzellen
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, SDS, ELISA, Crys
Crystallography grade
PVRL1
Spezies: Maus
Wirt: Insektenzellen
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, SDS, ELISA, Crys
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
PVRL1
(Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1))
Nectins are a small family of Ca -independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions. They are highly homologous to the human receptor for poliovirus, and as such have been alternately named poliovirus receptor-related proteins.Nectin-1 promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between Nectin-1 and Nectin-3 and between Nectin-1 and Nectin-4. Has some neurite outgrowth-promoting activity.
Molekulargewicht
36.9 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Tris-Bis PAGE result.