MCAM Protein (AA 24-559) (His tag)

Produktnummer ABIN7275297

Produktdetails

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Protein-Typ: Recombinant

Proteineigenschaft: AA 24-559

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses MCAM Protein ist gelabelt mit His tag.

Verwendungszweck: Human MUC18/CD146 Protein

Sequenz: Val24-Gly559

Produktmerkmale: Recombinant Human MUC18/CD146 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val24-Gly559.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human MUC18, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-MUC18, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Andere Bezeichnung: MUC18 (MCAM Produkte)

Synonyme: CD146 Protein, MUC18 Protein, Muc18 Protein, 1-gicerin Protein, AV025631 Protein, CD149 Protein, s-endo Protein, s-gicerin Protein, cd146 Protein, gicerin Protein, id:ibd3512 Protein, mcam Protein, MCAM Protein, melanoma cell adhesion molecule Protein, melanoma cell adhesion molecule b Protein, MCAM Protein, Mcam Protein, mcamb Protein

Hintergrund: METCAM/MUC18 is an integral membrane cell adhesion molecule (CAM) in the Ig-like gene super-family. It can carry out common functions of CAMs which is to perform intercellular interactions and interaction of cell with extracellular matrix in tumor microenvironment, to interact with various signaling pathways and to regulate general behaviors of cells.The above positive correlation is fortuitous, actually METCAM/MUC18 acts as a tumor and metastasis suppressor for the ovarian carcinoma cells.

Molekulargewicht: 60.8 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.

UniProt: P43121