MMP 9 (MMP9)
(Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))
Protein-Typ
Recombinant
Proteineigenschaft
AA 20-730
Spezies
Maus
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses MMP 9 Protein ist gelabelt mit His tag.
Verwendungszweck
Mouse MMP-9 Protein
Sequenz
Ala20-Pro730
Produktmerkmale
Recombinant Mouse MMP-9 Protein is expressed from HEK293 with His tag at the C-Terminus. The protein needs to be activated by APMA to have hydrolytic activity.It contains Ala20-Pro730.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Crystallography grade
MMP9
Spezies: Human
Wirt: Insektenzellen
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, SDS, ELISA, Crys
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
MMP 9 (MMP9)
(Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))
Matrix metalloproteinase 9 (MMP9) contributes to this process and deficiencies in the MMP9 lead to impaired healing. Inappropriate expression of MMP9 also contributes to impaired re-epithelialization. Previously we demonstrated that FOXO1 was activated in wound healing but to higher levels in diabetic wounds. To address mechanisms of impaired re-epithelialization we examined MMP9 expression in vivo in full thickness dermal scalp wounds created in experimental K14.
Molekulargewicht
79.64 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.