MAG Protein (AA 20-516) (His tag)
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- Target Alle MAG Proteine anzeigen
- MAG (Myelin Associated Glycoprotein (MAG))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 20-516
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Spezies
- Human
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses MAG Protein ist gelabelt mit His tag.
- Verwendungszweck
- Human Siglec-4a/MAG Protein
- Sequenz
- Gly20-Pro516
- Produktmerkmale
- Recombinant Human Siglec-4a/MAG Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly20-Pro516.
- Reinheit
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human Siglec-4a, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Siglec-4a Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA. See testing image for detail.
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS, 100 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- MAG (Myelin Associated Glycoprotein (MAG))
- Andere Bezeichnung
- Siglec-4a (MAG Produkte)
- Synonyme
- GMA Protein, S-MAG Protein, SIGLEC-4A Protein, SIGLEC4A Protein, siglec-4 Protein, MAG Protein, Gma Protein, siglec-4a Protein, myelin associated glycoprotein Protein, myelin-associated glycoprotein Protein, MAG Protein, mag Protein, Mag Protein
- Hintergrund
- Siglec-4a, also known as Myelin-Associated Glycoprotein (MAG), is a type I transmembrane glycoprotein belonging to the Siglec family, a subgroup of the Ig superfamily. Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2.
- Molekulargewicht
- 55.8 kDa. Due to glycosylation, the protein migrates to 75-82 kDa based on Tris-Bis PAGE result.
- UniProt
- P20916
- Pathways
- Neurotrophin Signalübertragung
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