LRRC15 Protein (His-Avi Tag)

Produktnummer ABIN7275215

Produktdetails

Target: LRRC15 (Leucine Rich Repeat Containing 15 (LRRC15))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses LRRC15 Protein ist gelabelt mit His-Avi Tag.

Sequenz: Tyr22-Gly538

Reinheit: > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human LRRC15, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-LRRC15 Antibody, hFc Tag with the EC₅₀ of 20.4ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: LRRC15 (Leucine Rich Repeat Containing 15 (LRRC15))

Andere Bezeichnung: LRRC15 (LRRC15 Produkte)

Synonyme: cb894 Protein, zgc:158286 Protein, LIB Protein, 5430427N11Rik Protein, Lib Protein, leucine rich repeat containing 15 Protein, leucine-rich repeat-containing protein 15 Protein, carboxypeptidase N subunit 2 Protein, LRRC15 Protein, lrrc15 Protein, CpipJ_CPIJ004946 Protein, CpipJ_CPIJ005191 Protein, CpipJ_CPIJ007745 Protein, CPN2 Protein, Lrrc15 Protein

Hintergrund: LIB, LRRC15,The chimeric protein binds in vitro and in vivo to a specific element upstream of LRRC15, leading to dramatic transcriptional activation.  LRRC15 encodes a leucine-rich transmembrane protein, present at the leading edge of migrating cells, the expression of which in normal tissues is restricted to the invasive cytotrophoblast layer of the placenta, small interfering (siRNA)-mediated suppression of LRRC15 expression in breast cancer cells leads to abrogation of invasiveness in vitro.

Molekulargewicht: 60.7 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.