IL-17A/F Protein (AA 24-155) (His tag)

Produktnummer ABIN7275001

Produktdetails

Target: IL-17A/F (Interleukin-17 A/F Heterodimer (IL-17A/F))

Protein-Typ: Recombinant

Proteineigenschaft: AA 24-155

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses IL-17A/F Protein ist gelabelt mit His tag.

Verwendungszweck: Human IL-17A & F Protein

Sequenz: Gly24-Ala155 (IL-17A) & Arg31-Gln163 (IL-17F)

Spezifität: Uni-Prot: Q16552-1 (IL-17A), Q96PD4 (IL-17F)

Produktmerkmale: Recombinant Human IL-17A & F Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly24-Ala155(IL-17A) & Arg31-Gln163(IL-17F).

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-17A&F, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 34.0ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: IL-17A/F (Interleukin-17 A/F Heterodimer (IL-17A/F))

Andere Bezeichnung: IL-17A&F (IL-17A/F Produkte)

Synonyme: il17-5 Protein, si:ch211-63o20.2 Protein, interleukin 17a/f3 Protein, il17a/f3 Protein

Hintergrund: Interleukin-17A (IL-17A), also known as CTLA-8, is a 15-20 kDa glycosylated cytokine that plays an important role in anti-microbial and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.

Molekulargewicht: 15.1 kDa (IL-17A) & 15.7 kDa (IL-17F). Due to glycosylation, the protein migrates to 16 kDa & 27 kDa based on Tris-Bis PAGE result.