GPA33 Protein (AA 22-235) (His-Avi Tag)

Produktnummer ABIN7274766

Produktdetails

Target: GPA33 (Glycoprotein A33 (Transmembrane) (GPA33))

Protein-Typ: Recombinant

Proteineigenschaft: AA 22-235

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses GPA33 Protein ist gelabelt mit His-Avi Tag.

Verwendungszweck: Human GPA33/A33 Protein

Sequenz: Ile22-Val235

Produktmerkmale: Recombinant Human GPA33/A33 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile22-Val235.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human GPA33, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-GPA33 Antibody, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: GPA33 (Glycoprotein A33 (Transmembrane) (GPA33))

Andere Bezeichnung: GPA33 (GPA33 Produkte)

Synonyme: A33 Protein, 2010310L10Rik Protein, 2210401D16Rik Protein, BB116197 Protein, glycoprotein A33 Protein, glycoprotein A33 (transmembrane) Protein, GPA33 Protein, Gpa33 Protein

Hintergrund: Glycoprotein A33 (GPA33) is also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95 % of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling.

Molekulargewicht: 26.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.