GFRA1 Protein (AA 25-424) (His tag)

Produktnummer ABIN7274741

Produktdetails

Target: GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 25-424

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses GFRA1 Protein ist gelabelt mit His tag.

Verwendungszweck: Human GFR alpha 1 Protein

Sequenz: Asp25-Ser424

Produktmerkmale: Recombinant Human GFR alpha 1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp25-Ser424.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human GDNF, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.7ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))

Andere Bezeichnung: GFR alpha 1 (GFRA1 Produkte)

Synonyme: gdnfr Protein, ret1l Protein, retl1 Protein, trnr1 Protein, gdnfra Protein, MGC122616 Protein, gfr-alpha-1 Protein, GDNFR Protein, GDNFRA Protein, GFR-ALPHA-1 Protein, RET1L Protein, RETL1 Protein, TRNR1 Protein, GDNFRALPHA Protein, GFRA1 Protein, AU042498 Protein, gfralpha1a Protein, zgc:110129 Protein, GDNF family receptor alpha 1 Protein, glial cell line derived neurotrophic factor family receptor alpha 1 Protein, gdnf family receptor alpha 1a Protein, gfra1 Protein, GFRA1 Protein, Gfra1 Protein, gfra1a Protein

Hintergrund: Neuroblastomas often undergo spontaneous differentiation and/or regression in vivo, which is at least partly regulated by the signals through neurotrophins and their receptors. Recently, glial cell line-derived neurotrophic factor (GDNF) and a second family member, neurturin (NTN), have been found to mediate their signals by binding to a heterotetrameric complex of c-Ret tyrosine kinase receptors and glycosylphosphatidylinositol-linked proteins, GFR alpha-1 (GDNFR-alpha) or GFR alpha-2 (TrnR2/GDNFR-beta/NTNR-alpha/RETL2).

Molekulargewicht: 45.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

NCBI Accession: NP_665736