TNFSF18 Protein (Trimer) (His-DYKDDDDK Tag)

Produktnummer ABIN7274681

Produktdetails

Target: TNFSF18 (Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18))

Protein-Typ: Recombinant

Proteineigenschaft: Trimer

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses TNFSF18 Protein ist gelabelt mit His-DYKDDDDK Tag.

Sequenz: Gln50-Ser177

Reinheit: > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human GITR Ligand Trimer, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human GITR, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: TNFSF18 (Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18))

Andere Bezeichnung: GITR Ligand (TNFSF18 Produkte)

Synonyme: Gitrl Protein, AITRL Protein, GITRL Protein, TL6 Protein, hGITRL Protein, TNLG2A Protein, tumor necrosis factor (ligand) superfamily, member 18 Protein, TNF superfamily member 18 Protein, Tnfsf18 Protein, TNFSF18 Protein

Hintergrund: TNFSF18, AITRL, TL6, GITRL, GITR Ligand,Glucocorticoid-induced TNFR-related protein (TNFRSF18, GITR, CD357), expressed by T cells, and its ligand (TNFSF18, GITRL), expressed by myeloid populations, provide co-stimulatory signals that boost T cell activity. Due to the important role that GITR plays in regulating immune functions, agonistic stimulation of GITR is a promising therapeutic concept.

Molekulargewicht: 47.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

UniProt: Q9UNG2