Fc gamma RII (CD32) (AA 32-215) protein (His tag)

Produktnummer ABIN7274654

Produktdetails

Target: Fc gamma RII (CD32)

Protein-Typ: Recombinant

Proteineigenschaft: AA 32-215

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: His tag

Verwendungszweck: Mouse Fc gamma RIII/CD16 Protein

Sequenz: Leu32-Thr215

Produktmerkmale: Recombinant Mouse Fc gamma RIII/CD16 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu32-Thr215.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: The affinity constant of 0.194 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Fc gamma RII (CD32)

Andere Bezeichnung: Fc gamma RIII (CD32 Produkte)

Synonyme: CD32 Protein, CD32B Protein, FCG2 Protein, FCGR2 Protein, IGFR2 Protein, FCGR2B Protein, AI528646 Protein, F630109E10Rik Protein, Fc[g]RII Protein, FcgRII Protein, Fcgr2 Protein, Fcgr2a Protein, Fcr-2 Protein, Fcr-3 Protein, Ly-17 Protein, Ly-m20 Protein, LyM-1 Protein, Lym-1 Protein, FCRII Protein, FcRII Protein, Fc fragment of IgG receptor IIb Protein, Fc receptor, IgG, low affinity IIb Protein, FCGR2B Protein, Fcgr2b Protein

Hintergrund: Immunoglobulin G (IgG) Fc receptors play a critical role in linking IgG antibody-mediated immune responses with cellular effector functions. A high resolution map of the binding site on human IgG1 for human Fc gamma RI, Fc gamma RIIA, Fc gamma RIIB, Fc gamma RIIIA, and FcRn receptors has been determined.A common set of IgG1 residues is involved in binding to all Fc gamma R, Fc gamma RII and Fc gamma RIII also utilize residues outside this common set.

Molekulargewicht: 22.2 kDa. Due to glycosylation, the protein migrates to 40-48 kDa based on Tris-Bis PAGE result.

UniProt: Q5D5I8