Fc epsilon RI/FCER1A (FCER1A)
(Fc Fragment of IgE Receptor Ia (FCER1A))
Protein-Typ
Recombinant
Proteineigenschaft
AA 26-205
Spezies
Human
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses Fc epsilon RI/FCER1A Protein ist gelabelt mit His tag.
Verwendungszweck
Human Fc epsilon RI alpha/FCER1a Protein
Sequenz
Val26-Gln205
Produktmerkmale
Recombinant Human Fc epsilon RI alpha/FCER1a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val26-Gln205.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human Fc epsilon RI alpha, His Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA. See testing image for detail.
SPR verified
FCER1A
Spezies: Cynomolgus
Wirt: HEK-293 Cells
Recombinant
>90 % as determined by SDS-PAGE.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
Fc epsilon RI/FCER1A (FCER1A)
(Fc Fragment of IgE Receptor Ia (FCER1A))
FCER1A Protein, FCE1A Protein, FcERI Protein, Iger01 Protein, RATIGER01 Protein, Fce1a Protein, Fcr-5 Protein, fcepsilonri Protein, Fc fragment of IgE receptor Ia Protein, Fc receptor, IgE, high affinity I, alpha polypeptide Protein, FCER1A Protein, Fcer1a Protein
Hintergrund
Known susceptibility genes to atopy and asthma have been identified by linkage or associations with clinical phenotypes, including total serum IgE levels. IgE-mediated sensitivity reactions require a high-affinity IgE receptor (FcepsilonRI), which immobilizes the immunoglobulin on the surface of the effector cells, mostly mast cells and basophils. Similarly to the previously investigated beta subunit of the receptor, FCER1A is a good candidate for a quantitative trait locus (QTL) in allergic diseases, and appears to participate in the systemic regulation of IgE levels.
Molekulargewicht
22.1 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Tris-Bis PAGE result.