AIMP1
(Aminoacyl tRNA Synthetase Complex-Interacting Multifunctional Protein 1 (AIMP1))
Protein-Typ
Recombinant
Proteineigenschaft
AA 2-312
Spezies
Human
Quelle
Escherichia coli (E. coli)
Aufreinigungstag / Konjugat
Dieses AIMP1 Protein ist gelabelt mit His tag.
Verwendungszweck
Human EMAP-II/AIMP1 Protein
Sequenz
Ala2-Lys312
Produktmerkmale
Recombinant Human EMAP-II/AIMP1 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Ala2-Lys312.
Reinheit
> 95 % as determined by Tris-Bis PAGE
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human EMAP-II, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-EMAP-II Antibody, hFc Tag with the EC50 of 0.18ug/ml determined by ELISA. See testing image for detail.
Crystallography grade
AIMP1
Spezies: Human
Wirt: Insektenzellen
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA, Crys
Crystallography grade
AIMP1
Spezies: Human
Wirt: Insektenzellen
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA, Crys
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
Supplied as 0.22μm filtered solution in 20 mM Tris, 500 mM NaCl, 10 % Glycerol ( pH 8.0).
Lagerung
-80 °C
Informationen zur Lagerung
Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
AIMP1
(Aminoacyl tRNA Synthetase Complex-Interacting Multifunctional Protein 1 (AIMP1))
The potent antiangiogenic and proinflammatory protein Endothelial Monocyte Activating Polypeptide II (EMAP II) has been described as a mediator of pulmonary vascular and alveolar formation and its expression is inversely related to the periods of vascularization and alveolarization in the developing lung. Hence the study of EMAP II could play a vital role in studying and devising appropriate therapeutics for diseases of aberrant lung development, such as BPD.
Molekulargewicht
35.4 kDa. The protein migrates to 40-45 kDa based on Tris-Bis PAGE result.