CLEC9A
(C-Type Lectin Domain Family 9, Member A (CLEC9A))
Protein-Typ
Recombinant
Proteineigenschaft
AA 57-241
Spezies
Human
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses CLEC9A Protein ist gelabelt mit Fc Tag.
Verwendungszweck
Human CLEC9A Protein
Sequenz
Lys57-Val241
Produktmerkmale
Recombinant Human CLEC9A Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Lys57-Val241.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human CLEC9A, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CLEC9A Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
Crystallography grade
CLEC9A
Spezies: Human
Wirt: Insektenzellen
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
ELISA, WB, SDS, Crys
CLEC9A
Spezies: Human
Wirt: Tobacco (Nicotiana tabacum)
Recombinant
>80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
ELISA, WB, SDS
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
CLEC9A
(C-Type Lectin Domain Family 9, Member A (CLEC9A))
9830005G06Rik Protein, DNGR-1 Protein, DNGR1 Protein, UNQ9341 Protein, RGD1562513 Protein, C-type lectin domain family 9, member a Protein, C-type lectin domain containing 9A Protein, C-type lectin domain family 9, member A Protein, Clec9a Protein, CLEC9A Protein
Hintergrund
CLEC9A expression was significantly higher in psoriatic skin compared with healthy donor. In psoriatic skin and PsA ST, CLEC9A() cells were in close proximity to TUNEL() cells. SF CLEC9A levels were significantly lower compared with paired PsA serum. Adalimumab treatment did not affect CLEC9A serum level and skin expression. The downregulation of synovial CLEC9A might be associated with a novel mechanism by which anti-TNF therapy might reduce CD8-mediated inflammation in PsA patients.
Molekulargewicht
48.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.