CDCP1 Protein (His tag)

Produktnummer ABIN7274293

Produktdetails

Target: CDCP1 (CUB Domain Containing Protein 1 (CDCP1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses CDCP1 Protein ist gelabelt mit His tag.

Sequenz: Phe30-Leu666

Reinheit: > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CDCP1 at 2μg/ml (100μl/Well). Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA. The affinity constant of 0.18nM as determined in SPR assay (Biacore T200). See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: CDCP1 (CUB Domain Containing Protein 1 (CDCP1))

Andere Bezeichnung: CDCP1 (CDCP1 Produkte)

Synonyme: CD318 Protein, SIMA135 Protein, TRASK Protein, 9030022E12Rik Protein, AA409659 Protein, E030027H19Rik Protein, RGD1305578 Protein, zgc:113071 Protein, CUB domain containing protein 1 Protein, CUB domain containing protein 1a Protein, CDCP1 Protein, Cdcp1 Protein, cdcp1a Protein

Hintergrund: CDCP1, CD318, SIMA135, TRASK, UNQ2486, PRO5773,Tumor metastasis depends on the dynamic regulation of cell adhesion through β1-integrin. The Cub-Domain Containing Protein-1, CDCP1, is a transmembrane glycoprotein which regulates cell adhesion. Overexpression and loss of CDCP1 have been observed in the same cancer types to promote metastatic progression.

Molekulargewicht: 72.8 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.