FCGR2A Protein (AA 28-208) (His tag)

Produktnummer ABIN7274277

Produktdetails

Target: FCGR2A (Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))

Protein-Typ: Recombinant

Proteineigenschaft: AA 28-208

Spezies: Cynomolgus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses FCGR2A Protein ist gelabelt mit His tag.

Verwendungszweck: Cynomolgus Fc gamma RIIA/CD32a Protein

Sequenz: Gln28-Pro208

Produktmerkmale: Recombinant Cynomolgus Fc gamma RIIA/CD32a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln28-Pro208.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: The affinity constant of 3.65 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: FCGR2A (Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))

Andere Bezeichnung: Fc gamma RIIA (FCGR2A Produkte)

Synonyme: CD32 Protein, CD32A Protein, CDw32 Protein, FCG2 Protein, FCGR2 Protein, FCGR2A1 Protein, FcGR Protein, IGFR2 Protein, CD32a Protein, Fc fragment of IgG receptor IIa Protein, FCGR2A Protein

Hintergrund: The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains, these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.

Molekulargewicht: 21.3 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.