CD47 Protein (CD47) (AA 19-139) (Fc Tag)
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- Target Alle CD47 Proteine anzeigen
- CD47
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 19-139
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Spezies
- Human
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses CD47 Protein ist gelabelt mit Fc Tag.
- Verwendungszweck
- Human CD47 Protein
- Sequenz
- Gln19-Pro139
- Produktmerkmale
- Recombinant Human CD47 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln19-Pro139.
- Reinheit
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human SIRP alpha, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 38.1ng/ml determined by ELISA. The affinity constant of 13.8nM as determined in SPR assay (Biacore T200). See testing image for detail.
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- CD47
- Andere Bezeichnung
- CD47 (CD47 Produkte)
- Synonyme
- IAP Protein, MER6 Protein, OA3 Protein, 9130415E20Rik Protein, AA407862 Protein, AI848868 Protein, AW108519 Protein, B430305P08Rik Protein, Itgp Protein, CD47/IAP Protein, alkaline phosphatase, intestinal Protein, CD47 molecule Protein, CD47 antigen (Rh-related antigen, integrin-associated signal transducer) Protein, Cd47 molecule Protein, ALPI Protein, CD47 Protein, Cd47 Protein
- Hintergrund
- CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.
- Molekulargewicht
- 40.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
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