TNFSF8 Protein (AA 63-234) (mFc Tag)

Produktnummer ABIN7274156

Produktdetails

Target: TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))

Protein-Typ: Recombinant

Proteineigenschaft: AA 63-234

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses TNFSF8 Protein ist gelabelt mit mFc Tag.

Verwendungszweck: Human CD30 Ligand/TNFSF8 Protein

Sequenz: Gln63-Asp234

Produktmerkmale: Recombinant Human CD30 Ligand/TNFSF8 Protein is expressed from HEK293 with mFc (IgG2a) tag at the N-Terminus.It contains Gln63-Asp234.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC,

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CD30, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Human CD30 Ligand, mFc (IgG2a) Tag with the EC50 of 31.9ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))

Andere Bezeichnung: CD30 Ligand (TNFSF8 Produkte)

Synonyme: CD153 Protein, CD30LG Protein, Cd30l Protein, CD30L Protein, TNFRSF8 Protein, TNFSF8 Protein, tumor necrosis factor (ligand) superfamily, member 8 Protein, TNF superfamily member 8 Protein, tumor necrosis factor superfamily member 8 Protein, Tnfsf8 Protein, TNFSF8 Protein

Hintergrund: CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells.CD30L is a cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.

Molekulargewicht: 46.2 kDa. Due to glycosylation, the protein migrates to 62-68 kDa based on Tris-Bis PAGE result.