CD34 Protein (CD34) (AA 32-290) (His tag)

Produktnummer ABIN7274141

Produktdetails

Target: CD34

Protein-Typ: Recombinant

Proteineigenschaft: AA 32-290

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses CD34 Protein ist gelabelt mit His tag.

Verwendungszweck: Human CD34 Protein

Sequenz: Ser32-Thr290

Produktmerkmale: Recombinant Human CD34 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser32-Thr290.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CD34, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD34 Antibody, hFc Tag with the EC50 of 16.5ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: CD34

Andere Bezeichnung: CD34 (CD34 Produkte)

Synonyme: AU040960 Protein, CD34 molecule Protein, CD34 antigen Protein, CD34 Protein, Cd34 Protein

Hintergrund: CD34 is a transmembrane phosphoglycoprotein, first identified on hematopoietic stem and progenitor cells. Clinically, it is associated with the selection and enrichment of hematopoietic stem cells for bone marrow transplants. Due to these historical and clinical associations, CD34 expression is almost ubiquitously related to hematopoietic cells, and it is a common misconception that CD34-positive (CD34()) cells in nonhematopoietic samples represent hematopoietic contamination.

Molekulargewicht: 28.6 kDa. Due to glycosylation, the protein migrates to 90-130 kDa based on Tris-Bis PAGE result.