HLA DPA1*02:02-DPB1*05:01 protein (V5 tag,SBP Tag,His tag)

Produktnummer ABIN7273278

Produktdetails

Target: HLA DPA1*02:02-DPB1*05:01

Protein-Typ: Recombinant

Spezies: Human

Quelle: Mammalian Cells

Aufreinigungstag / Konjugat: V5 tag,SBP Tag,His tag

Applikation: Blocking Peptide (BP), Control Peptide (CP), ELISA, Neutralization (Neut)

Verwendungszweck: The product is HLA DPA1*02:02-DPB1*05:01 as zipper-stabilized heterodimer

Sequenz: alpha chain: AGAIKADHVS TYAMFVQTHR PTGEFMFEFD EDEQFYVDLD KKETVWHLEE FGRAFSFEAQ GGLANIAILN NNLNTLIQRS NHTQAANDPP EVTVFPKEPV ELGQPNTLIC HIDRFFPPVL NVTWLCNGEP VTEGVAESLF LPRTDYSFHK FHYLTFVPSA EDVYDCRVEH WGLDQPLLKH WEAQEPIQ
beta chain: PENYLFQGRQ ECYAFNGTQR FLERYIYNRE ELVRFDSDVG EFRAVTELGR PEAEYWNSQK DILEEKRAVP DRMCRHNYEL DEAVTLQRRV QPKVNVSPSK KGPLQHHNLL VCHVTDFYPG SIQVRWFLNG QEETAGVVST NLIRNGDWTF QILVMLEMTP QQGDVYICQV EHTSLDSPVT VEWKAQSDSA RSKT
Sequences without tags. Tag location is at the discretion of the manufacturer.

Spezifität: HLA DPA1*02:02-DPB1*05:01, P20036 / P04440

Produktmerkmale: HLAs are produced in mammalian cell cultures as recombinant, truncated, soluble antigens, as zipper-stabilized heterodimers (HLA class II). As part of our quality control, all HLA are analyzed for purity by gel electrophoresis, concentration is determined by ELISA (0.5 mg/ml) and their functionality is verified with a specific antiserum.

Aufreinigung: Chromatography

Reinheit: > 80 %

Endotoxin-Niveau: Endotoxins have not been removed

Anwendungsinformationen

Applikationshinweise: ELISA: 10 - 1000 ng/mL

Kommentare:

sandwich ELISA, direct ELISA, bead ELISA, reactive with antibodies anti-V5 and anti-His and specific antiserum, B cell ELI Spot (predicted), in vitro memory B cell stimulation (predicted), HLA competition assay (predicted), control in various assays (predicted)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Phosphate based buffer, pH 7.8, Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Haltbarkeit: 6 months

Antigendetails

Target: HLA DPA1*02:02-DPB1*05:01

Hintergrund: Human Leukozyte Antigen, human Antigen-presenting major histocompatibility complex class II (MHCII) molecule. Expressed on Antigen-presenting cells (APC), displays peptides to CD4-positive T cells. Involved in cellular immune response.

Molekulargewicht: approx. 60 - 65 kDa (approx. 28 kDa alpha chain and 33 - 37 kDa beta chain)

UniProt: P20036