HLA DRA*01:01-DRB1*04:01 protein (V5 tag,SBP Tag,His tag)

Produktnummer ABIN7273234

Produktdetails

Target: HLA DRA*01:01-DRB1*04:01

Protein-Typ: Recombinant

Spezies: Human

Quelle: Mammalian Cells

Aufreinigungstag / Konjugat: V5 tag,SBP Tag,His tag

Applikation: Blocking Peptide (BP), Control Peptide (CP), ELISA, Neutralization (Neut)

Verwendungszweck: The product is HLA DRA*01:01-DRB1*04:01 as zipper-stabilized heterodimer

Sequenz: alpha chain: IKEEHVIIQA EFYLNPDQSG EFMFDFDGDE IFHVDMAKKE TVWRLEEFGR FASFEAQGAL ANIAVDKANL EIMTKRSNYT PITNVPPEVT VLTNSPVELR EPNVLICFID KFTPPVVNVT WLRNGKPVTT GVSETVFLPR EDHLFRKFHY LPFLPSTEDV YDCRVEHWGL DEPLLKHWEF DAPSPLPETT E
beta chain: GDTRPRFLEQ VKHECHFFNG TERVRFLDRY FYHQEEYVRF DSDVGEYRAV TELGRPDAEY WNSQKDLLEQ KRAAVDTYCR HNYGVGESFT VQRRVYPEVT VYPAKTQPLQ HHNLLVCSVN GFYPGSIEVR WFRNGQEEKT GVVSTGLIQN GDWTFQTLVM LETVPRSGEV YTCQVEHPSL TSPLTVEWRA RSESAQSK
Sequences without tags. Tag location is at the discretion of the manufacturer.

Spezifität: HLA DRA*01:01-DRB1*04:01, P01903 / P01911
HLA-DRB1*04:01 is associated with different diseases such as multiple sclerosis, rheumatoid arthritis or type 1 diabetes mellitus. People bearing HLA-DRB1*04:01 are more likely to be asymptomatic during an infection with SARS-CoV-2.

Produktmerkmale: HLAs are produced in mammalian cell cultures as recombinant, truncated, soluble antigens, as zipper-stabilized heterodimers (HLA class II). As part of our quality control, all HLA are analyzed for purity by gel electrophoresis, concentration is determined by ELISA (0.5 mg/ml) and their functionality is verified with a specific antiserum.

Aufreinigung: Chromatography

Reinheit: > 80 %

Endotoxin-Niveau: Endotoxins have not been removed

Anwendungsinformationen

Applikationshinweise: ELISA: 10 - 1000 ng/mL

Kommentare:

sandwich ELISA, direct ELISA, bead ELISA, reactive with antibodies anti-V5 and anti-His and specific antiserum, B cell ELI Spot (predicted), in vitro memory B cell stimulation (predicted), HLA competition assay (predicted), control in various assays (predicted)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Phosphate based buffer, pH 7.8, Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Haltbarkeit: 6 months

Antigendetails

Target: HLA DRA*01:01-DRB1*04:01

Hintergrund: Human Leukozyte Antigen, human Antigen-presenting major histocompatibility complex class II (MHCII) molecule. Expressed on Antigen-presenting cells (APC), displays peptides to CD4-positive T cells. Involved in cellular immune response.

Molekulargewicht: approx. 65 - 70 kDa (approx. 30 kDa alpha chain and 35 - 39 kDa beta chain)

UniProt: P01903