The in vivo grade recombinant rabbit IgG Fc protein (rIgG Fc, Cys 105 - Lys 323 of the constant region of the IgG heavy chain, Accession # P01870-1) was produced in mammalian cells and purified by protein A. The rIgG Fc protein includes the CH2 (114-213) and CH3 (222-323) domains of the heavy chain constant region as well as the hinge region (105-113) before the CH2 domain.
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
WB
Applikationshinweise
The recombinant rabbit IgG Fc protein and mutants can be used as the isotype control of the rIgG antibodies and the rIgG Fc fusion proteins in various in vitro and in vivo experiments.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
PBS, pH 7.4
Lagerung
-20 °C
Informationen zur Lagerung
1 month from date of receipt, 2 to 8°C as supplied. 3 months from date of receipt, -20°C to -70°C as supplied.
Haltbarkeit
3 months
Target
IgG Fc
Molekulargewicht
Predicted 24.8 kDa. Due to glycosylation, the recombinant rabbit IgG Fc protein migrates to 28-32 kDa based on the Bis-Tris PAGE result.