ZEBOV GP
(Zaire Ebola Virus Envelope Glycoprotein (ZEBOV GP))
Protein-Typ
Recombinant
Spezies
Ebola Virus
Quelle
Baculovirus infected Insect Cells
Aufreinigungstag / Konjugat
Dieses ZEBOV GP Protein ist gelabelt mit His tag.
Verwendungszweck
Recombinant EBOV (subtype Zaire, strain H.sapiens-wt/GIN/2014/Kissidougou-C15) Glycoprotein / GP Protein (His Tag)
Sequenz
Met1-Gln650
Produktmerkmale
A DNA sequence encoding the Zaire ebolavirus (strain H.sapiens-wt/GIN/2014/Kissidougou-C15) GP (AHX24649.1 ) (Met1-Gln650) was expressed with a polyhistidine tag at the C-terminus.
Reinheit
> 85 % as determined by SDS-PAGE
Endotoxin-Niveau
< 1.0 EU per μg protein as determined by the LAL method.
Please refer to the printed manual for detailed information.
Buffer
Lyophilized from sterile 20 mM Tris,500 mM NaCl,10 % glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Lagerung
4 °C,-20 °C,-80 °C
Informationen zur Lagerung
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Target
ZEBOV GP
(Zaire Ebola Virus Envelope Glycoprotein (ZEBOV GP))
The fourth gene of the EBOV genome encodes a 16- kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD), the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry, the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.