HCoV-OC43 N
(Coronavirus OC43 Nucleoprotein (HCoV-OC43 N))
Protein-Typ
Recombinant
Spezies
Human Coronavirus OC43 (HCoV-OC43)
Quelle
Escherichia coli (E. coli)
Aufreinigungstag / Konjugat
Dieses HCoV-OC43 N Protein ist gelabelt mit His tag.
Verwendungszweck
Recombinant Human coronavirus (HCoV-OC43) Nucleoprotein / NP Protein (His Tag)
Sequenz
Met1-Ile448
Produktmerkmale
A DNA sequence encoding the Human coronavirus (HCoV-OC43) Nucleoprotein (YP_009555245.1) (Met1-Ile448) was expressed with a polyhistide tag at the N-terminus.
HCoV-OC43 N
Spezies: Human Coronavirus OC43 (HCoV-OC43)
Wirt: Escherichia coli (E. coli)
Recombinant
Greater than 90 % as determined by reducing SDS-PAGE.
HCoV-OC43 N
Spezies: Human Coronavirus OC43 (HCoV-OC43)
Wirt: Escherichia coli (E. coli)
Recombinant
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Please refer to the printed manual for detailed information.
Buffer
Lyophilized from sterile 40 mM PB, 500 mM NaCl, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Lagerung
4 °C,-20 °C,-80 °C
Informationen zur Lagerung
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Target
HCoV-OC43 N
(Coronavirus OC43 Nucleoprotein (HCoV-OC43 N))
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.