VCAM1 Protein (His tag,Fc Tag)

Produktnummer ABIN7198677

Produktdetails

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses VCAM1 Protein ist gelabelt mit His tag,Fc Tag.

Verwendungszweck: Recombinant Mouse VCAM1 Protein (His & Fc Tag)(Active)

Sequenz: Met 1-Glu 698

Produktmerkmale: A DNA sequence encoding the extracellular domain (Met 1-Glu 698) of mouse VCAM1 (NP_035823.3) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Reinheit: > 90 % as determined by SDS-PAGE

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by the ability of the immobilized protein to support adhesion of U937 human histiocytic lymphoma cells. When cells are added to VCAM1-coated plates (10 μg/ml, 100 μg/well), approximately >70% cells will adhere specifically.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Andere Bezeichnung: VCAM1 (VCAM1 Produkte)

Synonyme: CD106 Protein, INCAM-100 Protein, Vcam-1 Protein, VCAM1B Protein, fi76d06 Protein, wu:fi76d06 Protein, zgc:158875 Protein, VCAM1 Protein, vascular cell adhesion molecule 1 Protein, vascular cell adhesion molecule 1b Protein, VCAM1 Protein, Vcam1 Protein, vcam1b Protein, VCAM-1 Protein

Hintergrund:

Background: Vascular cell adhesion molecule 1 (VCAM-1), also known as CD106, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. Two forms of VCAM-1 with either six or seven extracellular Ig-like domains are generated by alternative splicing, with the longer form predominant. VCAM-1 is an endothelial ligand for very late antigen-4 (VLA-4) and α4ß7 integrin expressed on leukocytes, and thus mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection, infection, and asthmatic responses. During these responses, VCAM-1 forms a scaffold for leukocyte migration. VCAM-1 also activates signals within endothelial cells resulting in the opening of an "endothelial cell gate" through which leukocytes migrate. VCAM-1 has been identified as a potential anti-inflammatory therapeutic target, the hypothesis being that reduced expression of VCAM-1 will slow the development of atherosclerosis. In addition, VCAM-1-activated signals in endothelial cells are regulated by cytokines indicating that it is important to consider both endothelial cell adhesion molecule expression and function during inflammatory processes.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Synonym: CD106;Vascular cell adhesion protein 1; Vcam1; L1CAM;VCMA1;Vcam-1

Molekulargewicht: 102 kDa

NCBI Accession: NP_035823

Pathways: Carbohydrate Homeostasis