UCHL1 Protein (His tag)

Produktnummer ABIN7198558

Produktdetails

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Ratte

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses UCHL1 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Rat UCHL1/PGP9.5 Protein (His Tag)(Active)

Sequenz: Glu 2-Ala 223

Produktmerkmale: A DNA sequence encoding the rat UCHL1 (Q00981) (Glu 2-Ala 223) was fused with a polyhistidine tag at the N-terminus.

Reinheit: > 97 % as determined by SDS-PAGE

Biological Activity Comment: Measured by the hydrolysis of UbiquitinAMC. The specific activity is >100 pmoles/min/μg.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Andere Bezeichnung: UCHL1/PGP9.5 (UCHL1 Produkte)

Synonyme: PARK5 Protein, PGP 9.5 Protein, PGP9.5 Protein, PGP95 Protein, Uch-L1 Protein, UCH-L1 Protein, UCHL1 Protein, cb358 Protein, wu:fc55h08 Protein, park5 Protein, pgp9.5 Protein, uch-l1 Protein, MGC132191 Protein, uchl1 Protein, AW822034 Protein, C88048 Protein, R75593 Protein, UCHL-1 Protein, gad Protein, ubiquitin C-terminal hydrolase L1 Protein, ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) Protein, ubiquitin C-terminal hydrolase L1 L homeolog Protein, ubiquitin carboxyl-terminal esterase L1 Protein, ubiquitin carboxy-terminal hydrolase L1 Protein, UCHL1 Protein, uchl1 Protein, uchl1.L Protein, LOC100304750 Protein, Uchl1 Protein

Hintergrund:

Background: Ubiquitin carboxyl-terminal hydrolase isozyme L1, also known as UCH-L1, Ubiquitin thioesterase L1, PGP9.5 and UCHL1, is a deubiqutinating enzyme with important functions in recycling of ubiquitin. Regulated proteolysis by the ubiquitin pathway has been implicated in control of the cell cycle, transcriptional activation, cell fate and growth, and synaptogenesis. The ubiquitin-proteasome system is involved in synaptic plasticity and is proposed to be part of a molecular switch that converts short-term synaptic potentiation to long-term changes in synaptic strength. UCHL1 is found in neuronal cell bodies and processes throughout the neocortex (at protein level). It is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. UCHL1 is weakly expressed in ovary. UCHL1 is a ubiquitin-protein hydrolase. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. UCHL1 dysfunction has been associated with neurodegeneration in Parkinson's, Alzheimer's, and Huntington's disease patients. Reduced UCHL1 function may jeopardize the survival of CNS neurons.

Synonym: UCHL1

Molekulargewicht: 26.2 kDa

UniProt: Q00981

Pathways: Feeding Behaviour