TNK2 Protein (GST tag)

Produktnummer ABIN7317144

Produktdetails

Target: TNK2 (tyrosine Kinase, Non-Receptor, 2 (TNK2))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Aufreinigungstag / Konjugat: Dieses TNK2 Protein ist gelabelt mit GST tag.

Verwendungszweck: Recombinant Human ACK1/TNK2 Protein (GST Tag)

Sequenz: Gly 110-Trp 476

Produktmerkmale: A DNA sequence encoding the amino acid (Gly 110-Trp 476) of human ACK1 isoform 1 (NP_005772.3) was expressed with the GST tag at the N-terminus.

Reinheit: > 90 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Frozen, Liquid

Buffer: Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol, 0.5 mM EDTA, 0.5 mM PMSF, 0.5 mM TCEP

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Antigendetails

Target: TNK2 (tyrosine Kinase, Non-Receptor, 2 (TNK2))

Andere Bezeichnung: ACK1/TNK2 (TNK2 Produkte)

Synonyme: ACK Protein, ACK-1 Protein, ACK1 Protein, p21cdc42Hs Protein, AW552477 Protein, Ack Protein, Cdgip Protein, Pyk1 Protein, ack1 Protein, ackb Protein, tnk2 Protein, tnk2b Protein, wu:fj16g04 Protein, zgc:154019 Protein, tyrosine kinase non receptor 2 Protein, tyrosine kinase, non-receptor, 2 Protein, tyrosine kinase, non-receptor, 1 Protein, TNK2 Protein, Tnk2 Protein, tnk1 Protein

Hintergrund:

Background: ACK1 (also known as ACK, TNK2, or activated Cdc42 kinase) is a structurally unique non-receptor tyrosine kinase that is expressed in diverse cell types. This downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. The ACK1 protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. ACK1 integrates signals from plethora of ligand-activated receptor tyrosine kinases (RTKs), for example, MERTK, EGFR, HER2 and PDGFR to initiate intracellular signaling cascades. It binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR. ACK1 transduces extracellular signals to cytosolic and nuclear effectors such as the protein kinase AKT/PKB and androgen receptor (AR), to promote cell survival and growth. ACK1 participates in tumorigenesis, cell survival, and migration. Gene amplification and overexpression of ACK1 were found in many cancer types such as those of the lung and prostate. Recently, four somatic missense mutations of ACK1, which occur in the N-terminal region, the C-lobe of the kinase domain, and the SH3 domain, were identified in cancer tissue samples.

Synonym: ACK,ACK-1,ACK1,p21cdc42Hs

Molekulargewicht: 68 kDa

NCBI Accession: NP_005772