TGM3 Protein (His tag)

Produktnummer ABIN7317463

Produktdetails

Target: TGM3 (Transglutaminase 3 (E Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM3))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Aufreinigungstag / Konjugat: Dieses TGM3 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human TGM3/Transglutaminase 3 Protein (His Tag)(Active)

Sequenz: Ala 2-Glu 693

Produktmerkmale: A DNA sequence encoding the human TGM3 (Q08188) (Ala 2-Glu 693) was expressed, with a polyhistidine tag at the N-terminus.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to cleave a synthetic peptide Benzyloxycarbonyl-Gln-Gly and NH2OH.The specific activity is > 450 pmoles/min/μg.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.5, 10 % gly

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: TGM3 (Transglutaminase 3 (E Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM3))

Andere Bezeichnung: TGM3/Transglutaminase 3 (TGM3 Produkte)

Synonyme: TGE Protein, TG(E) Protein, AI893889 Protein, RGD1561831 Protein, transglutaminase 3 Protein, transglutaminase 3, E polypeptide Protein, TGM3 Protein, Tgm3 Protein

Hintergrund:

Background: Transglutaminases (TGase) are a family of calcium-dependent acyl-transfer enzymes ubiquitously expressed in mammalian cells and responsible for catalyzing covalent cross-links between proteins or peptides. Transglutaminase 3 (TGM3) is a member of a family of Ca2+-dependent enzymes that catalyze covalent cross-linking reactions between proteins or peptides. TGM3 isoform is widely expressed and is important for epithelial barrier formation. It is a zymogen, requiring proteolysis for activity. Calcium-activated TGM3 can bind, hydrolyze, and is inhibited by GTP, despite lacking structural homology with other GTP binding proteins. TGM3 displays a diffuse cytoplasmic distribution in vitro consistent with its proposed role in the early phase of cornified cell envelope assembly in the cytoplasm. TGM3-driven specific isopeptide bonds between intermediate filaments and KAPs participate to the progressive scaffolding of the hair shaft. Additionally, TGM3 may be a novel prognostic biomarker for esophageal squamous cell carcinoma (ESCC).

Synonym: TGE

Molekulargewicht: 78.8 kDa

UniProt: Q08188