SMPD1 Protein (AA 1-631) (His tag)

Produktnummer ABIN7198101

Produktdetails

Target: SMPD1 (Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 1-631

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Aufreinigungstag / Konjugat: Dieses SMPD1 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human SMPD1/ASM Protein (aa 1-631, His Tag)(Active)

Sequenz: Met 1-Cys 631

Produktmerkmale: A DNA sequence encoding the full length of human SMPD1 isoform 1 (NP_000534.3) (Met 1-Cys 631) was expressed, fused with a polyhistidine tag at the C-terminus.

Reinheit: > 94 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to cleave. 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine (HNPPC). The specific activity is >1,000 pmol/min/μg.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 0.1 % OGP, 10 % glycerol

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: SMPD1 (Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1))

Andere Bezeichnung: SMPD1/ASM (SMPD1 Produkte)

Synonyme: ASM Protein, ASMASE Protein, NPD Protein, A-SMase Protein, Zn-SMase Protein, aSMase Protein, SMPD1 Protein, sphingomyelin phosphodiesterase 1 Protein, sphingomyelin phosphodiesterase 1, acid lysosomal Protein, sphingomyelin phosphodiesterase Protein, SMPD1 Protein, Smpd1 Protein, LOC5578088 Protein

Hintergrund:

Background: Sphingomyelin phosphodiesterase 1 (SMPD1) , also known as ASM ( acid sphingomyelinase ), is a member of the acid sphingomyelinase family of enzymes. Three isoforms have been identified, isoform 1 is 631 amino acids (aa) in length as the pro form, while Isoform 2 and isoform 3 have lost catalytic activity. The active SMPD1 isoform 1 contains one saposin B-type domain that likely interacts with sphingomyelin, and a catalytic region. Human SMPD1 is 86 % aa identical to mouse SMPD1. SMPD1 is a monomeric lysosomal enzyme that converts sphingomyelin (a plasma membrane lipid ) into ceramide through the removal of phosphorylcholine. This generates second messenger components that participate in signal transduction. Defects in SMPD1 are the cause of Niemann-Pick disease type A (NPA) and type B (NPB), also known as Niemann-Pick disease classical infantile form and Niemann-Pick disease visceral form. Niemann-Pick disease is a clinically and genetically heterogeneous recessive disorder. NPB has little if any neurologic involvement and patients may survive into adulthood.

Synonym: ASM,ASMASE,NPD

Molekulargewicht: 65 kDa

NCBI Accession: NP_000534