PRC1 Protein (His tag)

Produktnummer ABIN7317266

Produktdetails

Target: PRC1 (Protein Regulator of Cytokinesis 1 (PRC1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Aufreinigungstag / Konjugat: Dieses PRC1 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human PRC1 Protein (His Tag)

Sequenz: Met 1-Ser 620

Produktmerkmale: A DNA sequence encoding the human PRC1 isoform 1 (NP_003972.1) (Met 1-Ser 620) was expressed, with a polyhistidine tag at the N-terminus.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 20 % glycerol, 3 mM DTT

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: PRC1 (Protein Regulator of Cytokinesis 1 (PRC1))

Andere Bezeichnung: PRC1 (PRC1 Produkte)

Synonyme: cb212 Protein, prc1 Protein, prc1l Protein, zgc:55982 Protein, zgc:86918 Protein, MGC68845 Protein, zgc:55664 Protein, ase1 Protein, ASE1 Protein, D7Ertd348e Protein, protein regulator of cytokinesis 1 Protein, protein regulator of cytokinesis 1a Protein, protein regulator of cytokinesis 1 S homeolog Protein, protein regulator of cytokinesis 1b Protein, PRC1 Protein, prc1a Protein, prc1.S Protein, prc1b Protein, CNC07120 Protein, prc1 Protein, Prc1 Protein

Hintergrund:

Background: PRC1 (protein regulator of cytokinesis 1) is a key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. It is essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. PRC1 is required for KIF14 localization to the central spindle and midbody. It is also required to recruit PLK1 to the spindle. PRC1 stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. It is a homodimer and interacts with the C-terminal Rho-GAP domain and the basic region of RACGAP1. The interaction with RACGAP1 inhibits its GAP activity towards CDC42 in vitro, which may be required for maintaining normal spindle morphology. PRC1 also interacts separately via its N-terminal region with the C-terminus of CENPE, KIF4A and KIF23 during late mitosis. It interacts with KIF14, IF20A and PLK1.

Synonym: ASE1

Molekulargewicht: 74 kDa

NCBI Accession: NP_003972