PRSS8 Protein (His tag)

Produktnummer ABIN7197547

Produktdetails

Target: PRSS8 (Protease, serine, 8 (PRSS8))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses PRSS8 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human Prostasin/PRSS8 Protein (His Tag)(Active)

Sequenz: Ala 30-Arg 322

Produktmerkmale: A DNA sequence encoding the mature form of human Prss8 (NP_002764.1) (Ala 30-Arg 322) was fused with a signal peptide at the N-terminus and a polyhistidine-tag at the C-terminus.

Reinheit: > 97 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to cleave the fluorogenic peptide substrate Boc-QAR-AMC (R&D Systems, Catalog # ES014). The specific activity is >10 pmoles/min/μg.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: PRSS8 (Protease, serine, 8 (PRSS8))

Andere Bezeichnung: Prostasin/PRSS8 (PRSS8 Produkte)

Synonyme: CAP1 Protein, PROSTASIN Protein, prostasin Protein, 2410039E18Rik Protein, AI313909 Protein, C79772 Protein, fr Protein, mCAP1 Protein, protease, serine 8 Protein, protease, serine, 8 Protein, protease, serine 8 (prostasin) Protein, PRSS8 Protein, Prss8 Protein

Hintergrund:

Background: Prostasin (Prss8), also known as channel activating protease 1 (CAP1), is a trypsinlike serine peptidase, and plays important roles in epithelial physiology. It is originally purified as an active, soluble enzyme from human seminal fluid and is highly expressed in prostate, lung, kidney, salivary gland and pancreas. Prostasin is expressed as a glycosyl-phosphatidylinositol (GPI)-anchored membrane protein in prostate epithelial cells, and also exists as a secreted proteolytic enzyme possibly via tryptic cleavage of its COOH-terminal hydrophobic domain. Prostasin is found to activate the epithelial sodium channel (ENaC) which is tightly regulated and is critical for maintaining salt and fluid balance in the lung and kidney in both normal and pathological conditions. Accordingly, prostasin has been proposed as a target for therapeutic inhibition in cystic fibrosis. In addition, prostasin inhibits prostate and breast cancer cell invasion in vitro, suggesting a functional role as a suppressor of tumor invasion, as well as a regulator of gene expression during inflammation.

Synonym: CAP1,PROSTASIN

Molekulargewicht: 32.8 kDa

NCBI Accession: NP_002764