PDE4B Protein (GST tag,His tag)
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- Target Alle PDE4B Proteine anzeigen
- PDE4B (phosphodiesterase 4B, cAMP-Specific (PDE4B))
- Protein-Typ
- Recombinant
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Spezies
- Human
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Quelle
- Baculovirus infected Insect Cells
- Aufreinigungstag / Konjugat
- Dieses PDE4B Protein ist gelabelt mit GST tag,His tag.
- Verwendungszweck
- Recombinant Human PDE4B/DPDE4 Protein (His & GST Tag)
- Sequenz
- Met 1-Thr 564
- Produktmerkmale
- A DNA sequence encoding the human PDE4B isofrom 2 (NP_001032416.1) (Met 1-Thr 564) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
- Reinheit
- > 80 % as determined by reducing SDS-PAGE.
- Endotoxin-Niveau
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product PDE4B Protein
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile 50 mM Tris, 100 mM NaCl, 0.5 mM GSH, 10 % glycerol, 0.5 mM PMSF, pH 8.0
- Lagerung
- 4 °C,-20 °C,-80 °C
- Informationen zur Lagerung
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- PDE4B (phosphodiesterase 4B, cAMP-Specific (PDE4B))
- Andere Bezeichnung
- PDE4B/DPDE4 (PDE4B Produkte)
- Synonyme
- dpde4 Protein, pde4b5 Protein, pdeivb Protein, Dpde4 Protein, R74983 Protein, dunce Protein, PDE4/IVb Protein, Pde4B1 Protein, Pde4B2 Protein, Pde4B3 Protein, Pde4B4 Protein, DPDE4 Protein, PDE4B5 Protein, PDEIVB Protein, phosphodiesterase 4B Protein, phosphodiesterase 4B, cAMP specific Protein, phosphodiesterase 4B S homeolog Protein, pde4b Protein, PDE4B Protein, Pde4b Protein, pde4b.S Protein
- Hintergrund
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Background: cAMP-specific 3',5'-cyclic phosphodiesterase 4B, also known as PDE4B and DPDE4, is a member of the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. Cyclic nucleotide phosphodiesterases (PDEs) comprise a large family of enzymes that catalyze the hydrolysis of cAMP or cGMP and are implicated in various diseases. The crystal structures reveal a common scheme of inhibitor binding to the PDEs: (i) a hydrophobic clamp formed by highly conserved hydrophobic residues that sandwich the inhibitor in the active site; (ii) hydrogen bonding to an invariant glutamine that controls the orientation of inhibitor binding. A scaffold can be readily identified for any given inhibitor based on the formation of these two types of conserved interactions. These structural insights will enable the design of isoform-selective inhibitors with improved binding affinity and should facilitate the discovery of more potent and selective PDE inhibitors for the treatment of a variety of diseases. PDE4B / DPDE4 hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. It is expressed in brain, heart, lung and skeletal muscle. PDE4B / DPDE4 may be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents
Synonym: DPDE4;PDEIVB
- Molekulargewicht
- 92.2 kDa
- NCBI Accession
- NP_001032416
- Pathways
- Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Myometrial Relaxation and Contraction
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